Tylenol's Impact on Glutathione and Liver Damage What To Avoid When Taking Glutathione About Acetaminophen Overdose Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Acetaminophen Toxicity in Children: Diagnosis, Clinical Assessment, and Treatment of Acute Overingestion Consultant360 Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.