What Is Acetaminophen? Definition, Uses, Side Effects, and Risks Olympic Behavioral Health Is Tylenol Safe During Pregnancy? Understanding Risk Factors Seeking Health Tylenol's Impact on Glutathione and Liver Damage The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram Exposure to acetaminophen and all its metabolites upon 10, 15, and 20 mg kg intravenous acetaminophen in very preterm infants Pediatric Research Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.