Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Does ibuprofen deplete glutathione? Glutathione IV Benefits for Athletes: Performance Support AZ IV Medics Ibuprofen administration attenuates serum TNF levels, hepatic glutathione depletion, hepatic apoptosis and mouse mortality after Fas stimulation ScienceDirect Lyuh Esther Liposome Glutathione Direct Ultra X Set (12ea) In South Korea Esther Formula SodaGift A Practical Approach to Avoiding Drug Nutrient Depletions NBI