Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen induces liver injury and depletes glutathione in mice brain: Prevention by Moringa oleifera extract ScienceDirect Tylenol and Glutathione: A Closer Look At Their Relationship I'm Dr Ashley Mayer . I'm a practicing integrative physician, and also a mama to two great kiddos of my own! If you caught my post on Ibuprofen a few days ago, The Truth About Why Most Glutathione Supplements Don't Work! Synergy Institute Acupuncture & Chiropractic Tylenol, Autism, And The Glutathione Connection OC Integrative Medicine